Low energy indoor network : deployment optimisation

This article considers what the minimum energy indoor access point deployment is in order to achieve a certain downlink quality-of-service. The article investigates two conventional multiple-access technologies, namely: LTE-femtocells and 802.11n Wi-Fi. This is done in a dynamic multi-user and multi-cell interference network. Our baseline results are reinforced by novel theoretical expressions. Furthermore, the work underlines the importance of considering optimisation when accounting for the capacity saturation of realistic modulation and coding schemes. The results in this article show that optimising the location of access points both within a building and within the individual rooms is critical to minimise the energy consumption

Energy consumption of 4G cellular networks : a London case study

This paper presents the results of a joint investigation conducted between academia and industry. The investigation focused on modelling and reducing the energy expenditure of a 4G LTE network in a London case-study area, using data sets from an existing 3G network deployment. In the first part of the paper, different multi-cell network modelling approaches were compared, including: stochastic and linear geometry, hexagonal cell layout with wrap- around, and realistic network topology. The impact of terrain and clutter is also examined. It was found that they had a similar performance profile (80%) correlation, and a back-off factor to translate theoretical to measured results can account for most of the differences. The second part of the paper focuses on effective techniques that can be used to reduce energy consumption. Sleep mode and heterogeneous networks are combined and an ERG of 15-46% is achieved

An alternative test of tackling ability in rugby league players

This study investigated the relationship between 2 tests of tackling ability, muscle strength, and power in semiprofessional rugby league players. Thirty-one players, 19 first-grade and 12 second-grade, underwent tests of muscle strength (1-repetition-maximum bench press, chin-up, and squat) and power (plyometric push-up and countermovement jump). Tackling ability was assessed via video analysis of under-and over-the-ball tackle drills. The first-grade players had significantly greater scores in both the under-the-ball (P = .03, effect size [ES] = 0.84, 95% CI 0.07-1.50) and over-the-ball tackling-ability tests (P < .001, ES =1.86, 95% CI 0.83-2.52) than the second-grade players. A large, significant relationship was found between under- and over-the-ball tackling ability (r = .55, 95% CI .24-.76, P = .001). Lower-body strength (r = .37, 95% CI .02-.64, P = .04) was moderately associated with under-the-ball tackling ability, whereas over-the-ball tackling ability was moderately associated with plyometric push-up performance (r = .39, 95% CI .04-.65, P = .03). This study found that over-the-ball tackling ability was significantly associated with under-the-ball tackling in semiprofessional rugby league players. Furthermore, it was found that, compared with the second-grade players, the first-grade players had superior tackle ability in both tackle drills. In this study it was observed that plyometric push-up peak power was significantly related to over-the-ball tackling ability and absolute lower-body strength was associated with under-the-ball tackling ability. These findings provide skill coaches and strength and conditioning staff a greater understanding of elements that contribute to effective tackling ability

Canine pseudopregnancy: an evaluation of prevalence and current treatment protocols in the UK

Background: There is a dearth of literature on pseudopregnancy in the bitch, with only a few treatment-based studies published since the 1990s. Pseudopregnancy may be under-recognised in bitches and may account for a proportion of behavioural cases seen in veterinary practices including aggression. Little is known about commonly used treatments for overtly pseudopregnant bitches and it is possible that current regimes may not be prescribed for a sufficient duration to control any clinical signs including, physical and behavioural changes. To investigate current trends in diagnosis and treatment of canine pseudopregnancy, a postal survey was sent to 2000 randomly selected veterinary surgeons in UK veterinary practices. The questionnaire queried how often vets recognise cases of pseudopregnancy in spayed and entire bitches, which physical or behavioural signs are commonly recognised for diagnosis, and which management or treatment protocols are used. Results: The response rate was 19.8% (397/2000). Ninety-six percent of veterinary surgeons reported seeing pseudopregnant bitches showing behavioural changes without any physical changes within the last 12 months. Of those behavioural changes, collecting and mothering objects was the most frequently reported behavioural sign (96%). Ninety-seven percent of vets had seen aggression in pseudopregnant bitches. Nevertheless, only 52% of vets routinely asked owners about behavioural changes during consultations. Forty-nine percent of respondents reported seeing pseudopregnancy in spayed bitches. The most commonly reported physical sign was enlarged mammary glands and/or milk production (89%). Treatment options varied (surgical, medical or none) and depended on duration and severity of physical and behavioural signs, owners’ preference, cost, concurrent disease, drug availability and previous history. Conclusions: This is the largest epidemiological study of canine pseudopregnancy in the UK. The prevalence and severity of clinical signs in dogs with pseudopregnancy are variable and possibly under-estimated. Dogs with overt pseudopregnancy experience diverse physical and behavioural changes and information on standard treatment protocols are lacking. Although, progress on our understanding of diagnosis and treatment of pseudopregnancy in spayed and entire bitches has been made, further studies are warranted

Identification of Clinically Relevant Protein Targets in Prostate Cancer with 2D-DIGE Coupled Mass Spectrometry and Systems Biology Network Platform

Prostate cancer (PCa) is the most common type of cancer found in men and among the leading causes of cancer death in the western world. In the present study, we compared the individual protein expression patterns from histologically characterized PCa and the surrounding benign tissue obtained by manual micro dissection using highly sensitive two-dimensional differential gel electrophoresis (2D-DIGE) coupled with mass spectrometry. Proteomic data revealed 118 protein spots to be differentially expressed in cancer (n = 24) compared to benign (n = 21) prostate tissue. These spots were analysed by MALDI-TOF-MS/MS and 79 different proteins were identified. Using principal component analysis we could clearly separate tumor and normal tissue and two distinct tumor groups based on the protein expression pattern. By using a systems biology approach, we could map many of these proteins both into major pathways involved in PCa progression as well as into a group of potential diagnostic and/or prognostic markers. Due to complexity of the highly interconnected shortest pathway network, the functional sub networks revealed some of the potential candidate biomarker proteins for further validation. By using a systems biology approach, our study revealed novel proteins and molecular networks with altered expression in PCa. Further functional validation of individual proteins is ongoing and might provide new insights in PCa progression potentially leading to the design of novel diagnostic and therapeutic strategies

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Measurements of droplets from singing and some other activities

We present initial results from measurements of exhaled droplets by two female singers during singing, speaking, laughing, and recitation of poetry. We also conducted measurements with a flute, a clarinet, a tuba, and only the tuba's mouthpiece. This small project is motivated by the current distancing restrictions for choirs, musicians, and actors. To be able to image and track droplets, we have developed and built a portable measurement set-up. We have detected droplets with diameters of approximately 50 μm and above. We found that for a single subject, the largest amount of droplets are produced during singing, but that laughing can produce a comparable number of droplets. Speaking and recitation of poetry do not produce as many droplets. We repeated exercises at varying distances and found that the number of detected droplets decreased rapidly with increasing distance. However, the relative height difference between people, e.g. in a stage setting, must be considered when determining how far a droplet of a given size and velocity can reach. Most droplets we detected follow a ballistic trajectory, and hit the ground after a distance of approximately 1 m. In case of the musical instruments, we did not detect droplets exiting a tuba and only few single droplets from a flute and a clarinet. A clarinet usually points downwards, such that droplets follow a downward trajectory and hit the ground after a short distance. The situation was different when the tubist used only the mouthpiece (that is, detached from the tuba) during warm-up while partially blocking the opening with a finger: in this case, many droplets were ejected from the mouthpiece. These followed ballistic trajectories at different speeds, with some droplets exiting the measurement volume. Two important limitations of this study are that (i) the sample size is very small, such that a statistical analysis is beyond our scope; and (ii) that we cannot draw conclusions about a possible risk of infection with COVID-19 when performing any of the said activities.Norges KorforbundpublishedVersio